5 edition of Microtubule Protocols (Methods in Molecular Medicine) found in the catalog.
August 23, 2007
by Humana Press
Written in English
|The Physical Object|
|Number of Pages||305|
Owing to its indispensable role in cell division and in various cellular processes, the microtubule cytoskeleton is an attractive therapeutic target. Indeed, the microtubule-targeting agents are among the most effective drugs used in cancer chemotherapy and also show great promise for treating neurodegenerative disorders. Kinesin-like proteins (KLP) are thought to be responsible for transport of cellular cargoes and involved in many cellular processes. This book presents reproducible techniques for identification, purification, and characterization of the kinesin superfamily of microtubule-dependent motors.
Each tubulin molecule is a heterodimer, consisting of a subunit of α-tubulin and a subunit of β-tubulin. The dimers are arranged in linear rows called protofilaments. A microtubule usually consists of 13 protofilaments, arranged side by side, wrapped around the hollow core. This volume in the popular Methods in Molecular Biology(TM) series offers an up-to-date collection of methods and approaches for investigating microtubule dynamics in vitro and in cells. Includes material lists, step-by-step protocols and troubleshooting s in Molecular Biology: Microtubule Dynamics: Methods and Protocols (Hardcover).
Destructive Effects of Pulsed Electric Fields on Cancer Cells: The Microtubules Mechanical Resonance Clue. protocol defined in a recent book . Fluorescence Procedures for the Actin and Tubulin Cytoskeleton in Fixed Cells Actin: Louise Cramer Tubulin: Arshad Desai General Strategy We typically work with tissue culture, primary mammalian cells, and cell extracts, but the protocols can be adapted to other systems, such as whole embryos or lower eukaryotes. The cytoskeleton is veryFile Size: 94KB.
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Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. Microtubule Protocols presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting : Hardcover.
About this book Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space and mediate the transport of cargoes from the nucleus to the outer edges of the cell and : Humana Press.
Microtubules are at the heart of cellular self-organization, and their dynamic nature allows them to explore the intracellular space Microtubule Protocols book mediate the transport of cargoes from the nucleus to the outer edges of the cell and back.
Kinesin Protocols (methods In Molecular Biology) Download. By the end of the s only two microtubule-dependent motors, the plus end-directed kinesin and the minus end-directed cytoplasmic dynein, had been identified. Tubulin Protocols (Mitchison Lab) CBG. Notes: If taxol is added all at once it will cause tubulin precipitation.
If polymerizing 2 mg/ml tubulin, use 2 μM, 20 μM and μM steps. For DMSO polymerization it is best to have high tubulin concentrations ( File Size: KB.
Definitive and practical, Microtubule Dynamics: Methods and Protocols provides the key protocols needed by novices and experts on how to perform a broad range Microtubule Protocols book well-established and newly-emerging techniques in this vital : Hardcover.
Overview This book presents a comprehensive collection of essential and up-to-date methods for studying both the biology of microtubules and the mechanisms of action of microtubule-interacting drugs. The book contains a straightforward presentation of readily reproducible protocols, tips for troubleshooting, and advice on avoiding common : $ This unit describes an in vitro assay that uses video‐enhanced differential interference contrast (VE‐DIC) microscopy to examine the motile interactions between isolated organelle fractions and microtubules (MTs).
The method can be used to dissect the molecular requirements for organelle movement and membrane trafficking. ISOLATION OF MICROTUBULES.
All eukaryotic cells assemble microtubules, as they are the main structural components of the mitotic apparatus. However, the richest source of tubulin is terminally differentiated—and hence nondividing—neuronal tissue, because the axons and dendrites of neurons are full of assembled microtubules that can be readily depolymerized by Cited by: 1.
Summary. We present protocols for isolation of intestinal 3D structures from in vivo tissue and in vitro basement matrix embedded organoids, and detail different fixation and staining protocols optimized for immuno-labeling of microtubule, centrosomal, and junctional proteins as well as cell markers including the stem cell protein Lgr Cite this ArticleCited by: 1.
Microtubules display a very dynamic behavior, and the presence of the guanosine-triphosphate (GTP) cap at the plus ends of microtubules is essential to regulate microtubule dynamics. Dimitrov et al. () showed that GTP–tubulin is present not only at the plus ends but also in discrete locations along the microtubule lattice.
The microtubule-isolation procedures described here are based on the ability of the investigator to control the dimer-polymer distribution of tubulin by varying the temperature of the extract. In general, the extract is warmed to induce microtubule assembly, the polymer is collected by centrifugation, cooled to induce disassembly, clarified by.
Microtubule protocols (Book, )  Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to the cytoplasm of eukaryotic cells, some bacteria and some archaea (like Asgard).
A microtubule can grow as long as 50 micrometres and are highly dynamic. Wdr47 interacts with the Camsap family of microtubule minus-end-binding proteins; associates with microtubules through Camsap3, whose gene disruption also causes multi-axons; and promotes Camsap3 accumulation in neurites of unpolarized neurons.
Furthermore, Camsap overexpression rescues the polarization defects of Wdrdeficient neurons. Our. Microtubules, the third principal component of the cytoskeleton, are rigid hollow rods approximately 25 nm in diameter.
Like actin filaments, microtubules are dynamic structures that undergo continual assembly and disassembly within the cell. They function both to determine cell shape and in a variety of cell movements, including some forms of cell locomotion, the.
Microtubule bundling in cells. Affiliations. Department of Anatomy & Cell Biology, Columbia University, College of Physicians and Surgeons, New York, New York,USACited by: Microtubules are polymers of tubulin that form part of the cytoskeleton and provide structure and shape to the cytoplasm of eukaryotic cells, some bacteria and some archaea (like Asgard).
A microtubule can grow as long as 50 micrometres and are highly dynamic. TAXOL (Fig. 1) was isolated from the plant Taxus brevifolia (western yew) by Wani et al., who reported that the molecule has antitumour activity in several experimental systems1. In our laboratory Cited by: Microtubule targeting agents can be divided into two main groups based on their ability to depolymerise or hyperpolymerise microtubule filaments.
Such families of compounds include the taxanes and the vinca alkaloids which were first identified over 40 years ago and members of both drug families are currently in clinical use to treat a range of.
A schematic summarizing the activity of miRa in modulating the microtubule network in OS is presented in Figure 5D. Overall, our data present a novel mechanism of action of miRa in regulating STMN1 and microtubule stability and provides a compelling model for the involvement of miRa in augmenting the antitumor activity of MTAs in by: 6.Section V: Assay Protocol Microtubule assembly Microtubules (MTs) will be used as the substrate for your microtubule associated protein (MAP) assay.
You should use freshly prepared MTs within several hours. MTs should be kept at room temperature, this is very important as MTs will depolymerize on ice. Method 1.Viruses, an international, peer-reviewed Open Access journal.
Dear Colleagues, Microtubules are dynamic cytoskeletal filaments that participate in a vast and diverse array of cellular processes including transport of cargo and organelles, maintenance of organellar morphology and function, regulation of cell shape and motility and the distribution of genetic material and organelles at .